Other

Part:BBa_K3726113:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-08)


3CON5(H)_nNS5-down (PCC 11801)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 575
    Illegal PstI site found at 839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 575
    Illegal PstI site found at 839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 505
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 575
    Illegal PstI site found at 839
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 575
    Illegal PstI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON part the upstream overhang is CGCT, while the downstream overhang is AGCT.


Source

Homology connector has been found within the genome of Synechococcus elongatus PCC 11801.

Genome available at https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2219813